Journal: Cell Reports

Article Title: CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci

doi: 10.1016/j.celrep.2020.108545

Figure Lengend Snippet:

Article Snippet: Finally, cells were pooled and stained with anti-CD3 BV510 (Biolegend, #300448), anti-CD4 PE (Biolegend, #357404), anti-CD8 AF700 (Biolegend, #300920), anti-pSTAT1-Y701 AF647 (Cell Signaling, #8009S), anti-pSTAT1-S727 AF488 (Biolegend, #686410), anti-pSTAT3-Y705 AF488 (Biolegend, #651006) and anti-pSTAT3-S727 AF647 (Biolegend, #698913), anti-pSTAT4-Y693 AF488 (BD Biosciences, #558136), anti-pSTAT5-Y694 AF647 (Cell Signaling, #9365S), anti-pSTAT6-Y641 AF488 (BD Biosciences, #612600), anti-pERK-T202/Y204 AF488 (eBiosciences, #53-9109-41), anti-pAKT-S473 AF488 (Cell Signaling, #4071S), anti-pAKT-T308 AF647 (Cell Signaling, #48646S), anti-pP90RSK-S380 AF488 (Cell Signaling, #13588S), anti-pS6R-S240/S244 AF488 (Cell Signaling, #5018S), anti-pS6R-S235/S236 AF647 (Cell Signaling, #4851S), anti-pZAP70-Y319/pSYK-Y352 AF647 (Cell Signaling, #82975S), anti-pCREB-S133 AF488 (Cell Signaling, #9187S), anti-pHIS3-S10 AF647 (Cell Signaling, #9716S), anti-pGSK3β-S9 AF647 (Cell Signaling, #14332S),anti-pCFOS-S32 AF647 (Cell Signaling, #8677S), anti-IRF1 AF647 (Cell Signaling, #14105S), anti-IRF4 AF647 (Biolegend, #646408), anti-IRF7 AF647 (Biolegend, #656007), anti-GATA3 AF488 (Biolegend, #653807), anti-TBET AF647 (Biolegend, #644803), anti-HIF1α AF488 (Biolegend, #359707), anti-MYC AF488 (Cell Signaling, #12855S), anti-O-GlcNAC AF647 (NOVUS Biologicals, #NB300-524AF647), anti-STAT3 APC (BD Biosciences, #560392) and anti-PLCγ1 AF647 (BD Biosciences, #557883).

Techniques: Purification, Recombinant, Phospho-proteomics, Software

Journal: Cell Reports

Article Title: CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci

doi: 10.1016/j.celrep.2020.108545

Figure Lengend Snippet:

Article Snippet: Finally, cells were pooled and stained with anti-CD3 BV510 (Biolegend, #300448), anti-CD4 PE (Biolegend, #357404), anti-CD8 AF700 (Biolegend, #300920), anti-pSTAT1-Y701 AF647 (Cell Signaling, #8009S), anti-pSTAT1-S727 AF488 (Biolegend, #686410), anti-pSTAT3-Y705 AF488 (Biolegend, #651006) and anti-pSTAT3-S727 AF647 (Biolegend, #698913), anti-pSTAT4-Y693 AF488 (BD Biosciences, #558136), anti-pSTAT5-Y694 AF647 (Cell Signaling, #9365S), anti-pSTAT6-Y641 AF488 (BD Biosciences, #612600), anti-pERK-T202/Y204 AF488 (eBiosciences, #53-9109-41), anti-pAKT-S473 AF488 (Cell Signaling, #4071S), anti-pAKT-T308 AF647 (Cell Signaling, #48646S), anti-pP90RSK-S380 AF488 (Cell Signaling, #13588S), anti-pS6R-S240/S244 AF488 (Cell Signaling, #5018S), anti-pS6R-S235/S236 AF647 (Cell Signaling, #4851S), anti-pZAP70-Y319/pSYK-Y352 AF647 (Cell Signaling, #82975S), anti-pCREB-S133 AF488 (Cell Signaling, #9187S), anti-pHIS3-S10 AF647 (Cell Signaling, #9716S), anti-pGSK3β-S9 AF647 (Cell Signaling, #14332S),anti-pCFOS-S32 AF647 (Cell Signaling, #8677S), anti-IRF1 AF647 (Cell Signaling, #14105S), anti-IRF4 AF647 (Biolegend, #646408), anti-IRF7 AF647 (Biolegend, #656007), anti-GATA3 AF488 (Biolegend, #653807), anti-TBET AF647 (Biolegend, #644803), anti-HIF1α AF488 (Biolegend, #359707), anti-MYC AF488 (Cell Signaling, #12855S), anti-O-GlcNAC AF647 (NOVUS Biologicals, #NB300-524AF647), anti-STAT3 APC (BD Biosciences, #560392) and anti-PLCγ1 AF647 (BD Biosciences, #557883).

Techniques: Purification, Recombinant, Phospho-proteomics, Software

Journal: Cell Reports

Article Title: CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci

doi: 10.1016/j.celrep.2020.108545

Figure Lengend Snippet:

Article Snippet: Finally, cells were pooled and stained with anti-CD3 BV510 (Biolegend, #300448), anti-CD4 PE (Biolegend, #357404), anti-CD8 AF700 (Biolegend, #300920), anti-pSTAT1-Y701 AF647 (Cell Signaling, #8009S), anti-pSTAT1-S727 AF488 (Biolegend, #686410), anti-pSTAT3-Y705 AF488 (Biolegend, #651006) and anti-pSTAT3-S727 AF647 (Biolegend, #698913), anti-pSTAT4-Y693 AF488 (BD Biosciences, #558136), anti-pSTAT5-Y694 AF647 (Cell Signaling, #9365S), anti-pSTAT6-Y641 AF488 (BD Biosciences, #612600), anti-pERK-T202/Y204 AF488 (eBiosciences, #53-9109-41), anti-pAKT-S473 AF488 (Cell Signaling, #4071S), anti-pAKT-T308 AF647 (Cell Signaling, #48646S), anti-pP90RSK-S380 AF488 (Cell Signaling, #13588S), anti-pS6R-S240/S244 AF488 (Cell Signaling, #5018S), anti-pS6R-S235/S236 AF647 (Cell Signaling, #4851S), anti-pZAP70-Y319/pSYK-Y352 AF647 (Cell Signaling, #82975S), anti-pCREB-S133 AF488 (Cell Signaling, #9187S), anti-pHIS3-S10 AF647 (Cell Signaling, #9716S), anti-pGSK3β-S9 AF647 (Cell Signaling, #14332S),anti-pCFOS-S32 AF647 (Cell Signaling, #8677S), anti-IRF1 AF647 (Cell Signaling, #14105S), anti-IRF4 AF647 (Biolegend, #646408), anti-IRF7 AF647 (Biolegend, #656007), anti-GATA3 AF488 (Biolegend, #653807), anti-TBET AF647 (Biolegend, #644803), anti-HIF1α AF488 (Biolegend, #359707), anti-MYC AF488 (Cell Signaling, #12855S), anti-O-GlcNAC AF647 (NOVUS Biologicals, #NB300-524AF647), anti-STAT3 APC (BD Biosciences, #560392) and anti-PLCγ1 AF647 (BD Biosciences, #557883).

Techniques: Purification, Recombinant, Phospho-proteomics, Software

Journal: Cell Reports

Article Title: CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci

doi: 10.1016/j.celrep.2020.108545

Figure Lengend Snippet:

Article Snippet: Finally, cells were pooled and stained with anti-CD3 BV510 (Biolegend, #300448), anti-CD4 PE (Biolegend, #357404), anti-CD8 AF700 (Biolegend, #300920), anti-pSTAT1-Y701 AF647 (Cell Signaling, #8009S), anti-pSTAT1-S727 AF488 (Biolegend, #686410), anti-pSTAT3-Y705 AF488 (Biolegend, #651006) and anti-pSTAT3-S727 AF647 (Biolegend, #698913), anti-pSTAT4-Y693 AF488 (BD Biosciences, #558136), anti-pSTAT5-Y694 AF647 (Cell Signaling, #9365S), anti-pSTAT6-Y641 AF488 (BD Biosciences, #612600), anti-pERK-T202/Y204 AF488 (eBiosciences, #53-9109-41), anti-pAKT-S473 AF488 (Cell Signaling, #4071S), anti-pAKT-T308 AF647 (Cell Signaling, #48646S), anti-pP90RSK-S380 AF488 (Cell Signaling, #13588S), anti-pS6R-S240/S244 AF488 (Cell Signaling, #5018S), anti-pS6R-S235/S236 AF647 (Cell Signaling, #4851S), anti-pZAP70-Y319/pSYK-Y352 AF647 (Cell Signaling, #82975S), anti-pCREB-S133 AF488 (Cell Signaling, #9187S), anti-pHIS3-S10 AF647 (Cell Signaling, #9716S), anti-pGSK3β-S9 AF647 (Cell Signaling, #14332S),anti-pCFOS-S32 AF647 (Cell Signaling, #8677S), anti-IRF1 AF647 (Cell Signaling, #14105S), anti-IRF4 AF647 (Biolegend, #646408), anti-IRF7 AF647 (Biolegend, #656007), anti-GATA3 AF488 (Biolegend, #653807), anti-TBET AF647 (Biolegend, #644803), anti-HIF1α AF488 (Biolegend, #359707), anti-MYC AF488 (Cell Signaling, #12855S), anti-O-GlcNAC AF647 (NOVUS Biologicals, #NB300-524AF647), anti-STAT3 APC (BD Biosciences, #560392) and anti-PLCγ1 AF647 (BD Biosciences, #557883).

Techniques: Purification, Recombinant, Phospho-proteomics, Software

Journal: Cell Reports

Article Title: CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci

doi: 10.1016/j.celrep.2020.108545

Figure Lengend Snippet:

Article Snippet: Finally, cells were pooled and stained with anti-CD3 BV510 (Biolegend, #300448), anti-CD4 PE (Biolegend, #357404), anti-CD8 AF700 (Biolegend, #300920), anti-pSTAT1-Y701 AF647 (Cell Signaling, #8009S), anti-pSTAT1-S727 AF488 (Biolegend, #686410), anti-pSTAT3-Y705 AF488 (Biolegend, #651006) and anti-pSTAT3-S727 AF647 (Biolegend, #698913), anti-pSTAT4-Y693 AF488 (BD Biosciences, #558136), anti-pSTAT5-Y694 AF647 (Cell Signaling, #9365S), anti-pSTAT6-Y641 AF488 (BD Biosciences, #612600), anti-pERK-T202/Y204 AF488 (eBiosciences, #53-9109-41), anti-pAKT-S473 AF488 (Cell Signaling, #4071S), anti-pAKT-T308 AF647 (Cell Signaling, #48646S), anti-pP90RSK-S380 AF488 (Cell Signaling, #13588S), anti-pS6R-S240/S244 AF488 (Cell Signaling, #5018S), anti-pS6R-S235/S236 AF647 (Cell Signaling, #4851S), anti-pZAP70-Y319/pSYK-Y352 AF647 (Cell Signaling, #82975S), anti-pCREB-S133 AF488 (Cell Signaling, #9187S), anti-pHIS3-S10 AF647 (Cell Signaling, #9716S), anti-pGSK3β-S9 AF647 (Cell Signaling, #14332S),anti-pCFOS-S32 AF647 (Cell Signaling, #8677S), anti-IRF1 AF647 (Cell Signaling, #14105S), anti-IRF4 AF647 (Biolegend, #646408), anti-IRF7 AF647 (Biolegend, #656007), anti-GATA3 AF488 (Biolegend, #653807), anti-TBET AF647 (Biolegend, #644803), anti-HIF1α AF488 (Biolegend, #359707), anti-MYC AF488 (Cell Signaling, #12855S), anti-O-GlcNAC AF647 (NOVUS Biologicals, #NB300-524AF647), anti-STAT3 APC (BD Biosciences, #560392) and anti-PLCγ1 AF647 (BD Biosciences, #557883).

Techniques: Purification, Recombinant, Phospho-proteomics, Software

Journal: Cell Reports

Article Title: CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci

doi: 10.1016/j.celrep.2020.108545

Figure Lengend Snippet:

Article Snippet: Finally, cells were pooled and stained with anti-CD3 BV510 (Biolegend, #300448), anti-CD4 PE (Biolegend, #357404), anti-CD8 AF700 (Biolegend, #300920), anti-pSTAT1-Y701 AF647 (Cell Signaling, #8009S), anti-pSTAT1-S727 AF488 (Biolegend, #686410), anti-pSTAT3-Y705 AF488 (Biolegend, #651006) and anti-pSTAT3-S727 AF647 (Biolegend, #698913), anti-pSTAT4-Y693 AF488 (BD Biosciences, #558136), anti-pSTAT5-Y694 AF647 (Cell Signaling, #9365S), anti-pSTAT6-Y641 AF488 (BD Biosciences, #612600), anti-pERK-T202/Y204 AF488 (eBiosciences, #53-9109-41), anti-pAKT-S473 AF488 (Cell Signaling, #4071S), anti-pAKT-T308 AF647 (Cell Signaling, #48646S), anti-pP90RSK-S380 AF488 (Cell Signaling, #13588S), anti-pS6R-S240/S244 AF488 (Cell Signaling, #5018S), anti-pS6R-S235/S236 AF647 (Cell Signaling, #4851S), anti-pZAP70-Y319/pSYK-Y352 AF647 (Cell Signaling, #82975S), anti-pCREB-S133 AF488 (Cell Signaling, #9187S), anti-pHIS3-S10 AF647 (Cell Signaling, #9716S), anti-pGSK3β-S9 AF647 (Cell Signaling, #14332S),anti-pCFOS-S32 AF647 (Cell Signaling, #8677S), anti-IRF1 AF647 (Cell Signaling, #14105S), anti-IRF4 AF647 (Biolegend, #646408), anti-IRF7 AF647 (Biolegend, #656007), anti-GATA3 AF488 (Biolegend, #653807), anti-TBET AF647 (Biolegend, #644803), anti-HIF1α AF488 (Biolegend, #359707), anti-MYC AF488 (Cell Signaling, #12855S), anti-O-GlcNAC AF647 (NOVUS Biologicals, #NB300-524AF647), anti-STAT3 APC (BD Biosciences, #560392) and anti-PLCγ1 AF647 (BD Biosciences, #557883).

Techniques: Purification, Recombinant, Phospho-proteomics, Software

Journal: Cell Reports

Article Title: CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci

doi: 10.1016/j.celrep.2020.108545

Figure Lengend Snippet:

Article Snippet: Finally, cells were pooled and stained with anti-CD3 BV510 (Biolegend, #300448), anti-CD4 PE (Biolegend, #357404), anti-CD8 AF700 (Biolegend, #300920), anti-pSTAT1-Y701 AF647 (Cell Signaling, #8009S), anti-pSTAT1-S727 AF488 (Biolegend, #686410), anti-pSTAT3-Y705 AF488 (Biolegend, #651006) and anti-pSTAT3-S727 AF647 (Biolegend, #698913), anti-pSTAT4-Y693 AF488 (BD Biosciences, #558136), anti-pSTAT5-Y694 AF647 (Cell Signaling, #9365S), anti-pSTAT6-Y641 AF488 (BD Biosciences, #612600), anti-pERK-T202/Y204 AF488 (eBiosciences, #53-9109-41), anti-pAKT-S473 AF488 (Cell Signaling, #4071S), anti-pAKT-T308 AF647 (Cell Signaling, #48646S), anti-pP90RSK-S380 AF488 (Cell Signaling, #13588S), anti-pS6R-S240/S244 AF488 (Cell Signaling, #5018S), anti-pS6R-S235/S236 AF647 (Cell Signaling, #4851S), anti-pZAP70-Y319/pSYK-Y352 AF647 (Cell Signaling, #82975S), anti-pCREB-S133 AF488 (Cell Signaling, #9187S), anti-pHIS3-S10 AF647 (Cell Signaling, #9716S), anti-pGSK3β-S9 AF647 (Cell Signaling, #14332S),anti-pCFOS-S32 AF647 (Cell Signaling, #8677S), anti-IRF1 AF647 (Cell Signaling, #14105S), anti-IRF4 AF647 (Biolegend, #646408), anti-IRF7 AF647 (Biolegend, #656007), anti-GATA3 AF488 (Biolegend, #653807), anti-TBET AF647 (Biolegend, #644803), anti-HIF1α AF488 (Biolegend, #359707), anti-MYC AF488 (Cell Signaling, #12855S), anti-O-GlcNAC AF647 (NOVUS Biologicals, #NB300-524AF647), anti-STAT3 APC (BD Biosciences, #560392) and anti-PLCγ1 AF647 (BD Biosciences, #557883).

Techniques: Purification, Recombinant, Phospho-proteomics, Software

Journal: Cell Reports

Article Title: CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci

doi: 10.1016/j.celrep.2020.108545

Figure Lengend Snippet:

Article Snippet: Finally, cells were pooled and stained with anti-CD3 BV510 (Biolegend, #300448), anti-CD4 PE (Biolegend, #357404), anti-CD8 AF700 (Biolegend, #300920), anti-pSTAT1-Y701 AF647 (Cell Signaling, #8009S), anti-pSTAT1-S727 AF488 (Biolegend, #686410), anti-pSTAT3-Y705 AF488 (Biolegend, #651006) and anti-pSTAT3-S727 AF647 (Biolegend, #698913), anti-pSTAT4-Y693 AF488 (BD Biosciences, #558136), anti-pSTAT5-Y694 AF647 (Cell Signaling, #9365S), anti-pSTAT6-Y641 AF488 (BD Biosciences, #612600), anti-pERK-T202/Y204 AF488 (eBiosciences, #53-9109-41), anti-pAKT-S473 AF488 (Cell Signaling, #4071S), anti-pAKT-T308 AF647 (Cell Signaling, #48646S), anti-pP90RSK-S380 AF488 (Cell Signaling, #13588S), anti-pS6R-S240/S244 AF488 (Cell Signaling, #5018S), anti-pS6R-S235/S236 AF647 (Cell Signaling, #4851S), anti-pZAP70-Y319/pSYK-Y352 AF647 (Cell Signaling, #82975S), anti-pCREB-S133 AF488 (Cell Signaling, #9187S), anti-pHIS3-S10 AF647 (Cell Signaling, #9716S), anti-pGSK3β-S9 AF647 (Cell Signaling, #14332S),anti-pCFOS-S32 AF647 (Cell Signaling, #8677S), anti-IRF1 AF647 (Cell Signaling, #14105S), anti-IRF4 AF647 (Biolegend, #646408), anti-IRF7 AF647 (Biolegend, #656007), anti-GATA3 AF488 (Biolegend, #653807), anti-TBET AF647 (Biolegend, #644803), anti-HIF1α AF488 (Biolegend, #359707), anti-MYC AF488 (Cell Signaling, #12855S), anti-O-GlcNAC AF647 (NOVUS Biologicals, #NB300-524AF647), anti-STAT3 APC (BD Biosciences, #560392) and anti-PLCγ1 AF647 (BD Biosciences, #557883).

Techniques: Purification, Recombinant, Phospho-proteomics, Software